21) [46] and [47]. Mutational studies also indicated that the first arginine (R1), fourth proline (P4) and fifth asparate

(D5) were important for binding. Because the surface of titanium is covered with an oxide film TGF-beta inhibitor displaying both positively and negatively charged hydroxyl groups under physiological conditions, electrostatic interactions between –O− and R1, and –OH2+ and D5, have been proposed to underlie the interaction between minTBP-1 and titanium. Four kinds of peptide were prepared as summarized in Table 3. Adsorption assay of the synthesized peptides was carried out on crystal quartz sensors coated with Ti using a QCM-D instrument. The results demonstrated that accretion of surfactant reduced nonspecific interactions, dramatically enhancing the selectivity

and specificity of the Ti-binding peptides, and ensuring reversible specific binding (Fig. 22). In addition, the bioactivities GDC-973 of P. gingivalis cells on peptide-modified titanium were evaluated by ATP-bioluminescent assay. The bioactivity test revealed that ATP activity in P. gingivalis in peptide-modified specimens significantly decreased compared to that in the Ti control [45]. These findings indicate that surface modification with conjugated molecules consisting of antimicrobial and titanium-binding peptides is a promising method for reduction of biofilm formation on titanium implants. In conclusion, a cold-plasma surface modification is useful in controlling the physicochemical nature of surfaces, including the surface energy and the surface electrical

charge, leading to immobilize the drugs and peptides, and in developing bio-functional implants (Fig. 4). Considering HSP90 the present technology, it may be possible to produce implants with highly controlled surfaces that maintain homeostasis. The authors have no financial relationship with the organization that sponsored the research. This research was supported by an Oral Health Science Center Grant hrc7 and hrc8 from Tokyo Dental College, a “High-Tech Research Center” Project for Private Universities: Matching Fund Subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan, 2006–2010 and 2010–2012, and a Grant-in-Aid for Scientific Research (B:18390524 and 18659581) from the Japan Society for the Promotion of Science. “
“Dental caries is the most common pathological change of dentin. Fusayama’s research demonstrated that carious dentin consists of two distinct layers: an outer layer of bacterially infected dentin, and an inner layer of affected dentin [1]. The outer layer (caries-infected dentin) was characterized as being highly demineralized, physiologically unreminerazable and showing irreversible denatured collagen fibrils with a virtual disappearance of cross-linkages.