With disease progression, cardiac output (CO) cannot be further increased resulting in arterial hypotension, stimulation of the sympathetic nervous and renin-angiotensin

system as well as ascites. Tense ascites decreases venous return and thus CO due to compression of the inferior vena cava and right atrium. Finally, hepatorenal syndrome (HRS) is the extreme expression of this hemodynamic dysfunction. Therapeutic paracentesis acutely increases CO which has been previously identified to be an independent risk factor for the development SB203580 ic50 of HRS. However, the determination of CO is difficult in clinical routine due to invasive, operator dependent or time-consuming standard procedures such as right heart catheterization, echocardiography or cardiac magnetic resonance imaging. The aim of our study was to evaluate hemodynamic changes during paracentesis using non-invasive inert gas rebreathing (IGR). Methods: Routine therapeutic paracentesis was performed in the supine position using ultrasound guidance in 28 patients with tense ascites refractory to therapy. In patients with a volume of ascites removed (VA) > 5 liters albumin was administered. Hemodynamic parameters including CO, stroke volume (SV), heart rate

Epacadostat supplier (HR), systolic and diastolic blood pressure (SBP, DBP) were assessed immediately prior to and after Protein kinase N1 the procedure using IGR. Results: The collective (19 men) aged from 42 to 76 years. Mean MELD score was 13 with 15 patients in Child-Pugh class B (CPC) and 13 in C. Most frequent causes of cirrhosis were alcohol (16) and HCV (4) or HBV (3). Mean VA was 4400±1500 ml (range 1900 to 8000 ml). CO significantly increased from 5.7±1.7 to 7.0±2.0 l/min after paracentesis

(p<0.001). SV accordingly increased from 81±26 ml to 97±33 ml (p<0.001). Both SBP and DBP significantly dropped from 122±19 to 117±18 mmHg (p=0.04) and 69±12 to 63±13 mmHg (p<0.001). HR remained unchanged at 73±14 and 74±15 mmHg (p=0.69).There was a moderate correlation between VA and change in CO (r=0.36, p=0.12). We neither found differences between change in CO and CPC (p=0.31) nor the cause of cirrhosis (p=0.25). Conclusion: IGR is safe and feasible in tracking hemodynamic changes non-in-vasively induced by therapeutic paracentesis. Hyperdynamic circulation further increases acutely showing a moderate association with VA. Further studies are warranted as knowledge of hemodynamics may be beneficial in evaluating patients at risk for e.g. HRS, portopulmonary hypertension or hepatopul-monary syndrome. Disclosures: Christoph Antoni – Speaking and Teaching: Roche, MSD, BMS, Janssen, Gilead, Falk Foundation The following people have nothing to disclose: Joachim Saur, Thomas Zimmerer, Nenad Suvajac, Julia D.