Further, they do not report whether azygospore formation was observed. Nemoto and Aoki (1975) report of azygospores budding from clavate hyphal bodies of E. floridana in the spider mite O. hondoensis and they could not find binucleate zygospores. Ishikawa (2010) observed formation of azygospores by Neozygites sp. (N. tetranychi or N. floridana) in the spider mite host T. kanzawai. Humber (2012) states that in Neozygitomycetes

mature resting spores (zygospores) may have two adjacent round fenestrae (‘holes’ in the episporium) that raise a ridge of gametangial wall remnant between them. This supports our findings of remnants from the attachment of hyphal body/bodies to the resting spore both for the Norwegian and the Brazilian strains, in both immature and mature resting spores. Generally less distinct hyphal remnants CH5424802 purchase were observed for the Brazilian strain

than for the Norwegian strain ( Figs. 2D and F–G and 3F–H). For some of the remnants on the resting spore of the Norwegian strains it looks like only one hyphal body might have been attached to the spore, and we therefore suggest that these might be azygospores ( Fig. 3F), while, as mentioned in Humber (1981) and earlier in this paper, the doubled gametangial remnants on other spores suggest that two hyphal bodies were attached to the spore and that these spores are probably zygospores ( Fig. 3G and H). Weiser (1968) describes that in some cases there were a collar of remnants of the hypha around Ibrutinib cost the

round suture of the scar (azygospores) of T. tetranychi in the spider mite host T. athaeae. His illustrations look similar to the Brazilian strain with rather indistinct remnants. We further document immature azygospores with 1–3 nuclei (Norwegian strains), immature resting spores (probably azygospores) Sclareol with 1–8 nuclei (Brazilian strain) and mature resting spores with two nuclei (Norwegian and Brazilian strains, azygo- or zygospores). Weiser (1968) describes two nuclei inside mature azygospores of the fungus T. tetranychi, which is close to N. floridana, in T. althaeae. Also according to Humber, 1989, Keller, 1991, Keller, 1997 and Keller and Petrini, 2005, zygospores in Neozygites are binucleate. We observed that hyphal bodies in the mites normally had four nuclei and that one nucleus might be transferred to the budding azygospore ( Fig. 2C). Keller (1997) described that the cells of neozygitoid fungi exert strong control over nuclear number and, perhaps most significantly, a round of mitosis in gametangia immediately preceding conjugation and zygosporogenesis. However, Delalibera et al. (2004) observed that zygosporogenesis in N. tanajoae is preceded by reduction in nuclei number from the usual 3–4 to only two nuclei in gametangial cells. Our observations seems to correspond well with the results found by McCabe et al.