However, its popularity has declined essentially because of the variable vessel anatomy. The main disadvantage of the DCIA flap is that
its dissection is time consuming and requires a greater anatomical knowledge compared with other commonly harvested free flaps. Here, this website we describe the anatomical variability relevant to the DCIA flap to allow a clear and easy dissection.
Materials and Methods: Three male and seven female preserved Korean adult cadavers were dissected bilaterally (20 sides). The age at death ranged from 46 to 84 years. Various measurements were made, including the origins of the DCIA and deep circumflex iliac vein (DCIV), lengths of the DCIA and DCIV, and the types of ascending branch of the DCIA.
Results: The origin of the DCIA was 5.30 +/- 6.22 mm (mean +/- SD) superior to the inguinal ligament, and the DCIV was 4.75 +/- 3.14 mm medial to the origin of the DCIA. The length of the DCIA from its origin to the level of the anterior superior iliac spine was 59.35 +/- 9.06 mm, and the vertical distance between the anterior superior iliac spine and DCIA was 18.50 +/- 3.82mm. With regard to the branching pattern of ascending selleck chemicals branch, most cases (n = 18, 90%) exhibited 1 origin and 2 branches, and the remaining 2 cases (10%) had 2 origins and 2 branches. The distance from the DCIA origin to the branch point
in cases exhibiting 1 origin and 2 branches was 36.83 +/- 16.10 mm.
Conclusions: The anatomical findings presented here regarding anatomical variability relevant to DCIA flap harvesting
may facilitate the DCIA flap approach for clinicians.”
“Aim:
To https://www.selleckchem.com/products/ly2606368.html compare different outcomes of vitrification and slow freezing of isolated pre-antral follicles and to evaluate different cryo-devices for vitrification of isolated follicles.
Methods:
Pre-antral follicles were isolated from mouse ovaries and cryopreserved using vitrification and slow freezing. A preliminary experiment was carried out to select the optimal cryo-device for vitrification of isolated follicles. A total of 414 follicles were randomly distributed among four groups: control (CT) fresh (n = 100), nylon mesh (n = 96), electron microscopy grid (n = 102), and micro-capillary tips (n = 116). Subsequently, a total of 979 follicles were randomly assigned to three different groups: CT fresh (n = 256), vitrification (n = 399) and slow freezing (n = 324). CT and cryopreserved/thawed follicles were cultured in vitro and examined daily for development. Final maturation was triggered with human chorionic gonadotrophin and rates of oocyte maturation were calculated. The ultra-structure of cryopreserved/thawed follicles was studied using electron microscopy. Meiotic spindle presence and organization in mature oocytes were examined using the Oosight imaging system.