In addition, in Sprague Dawley rats antepartum maternal behavior, INCB024360 which was decreased as a result of PNS, was decreased in the granddaughters of the prenatally stress rats as well ( Ward et al., 2013). In guinea pigs transgenerational

effects on the HPA-axis function of PNS were shown; F2 offspring of PNS guinea pigs were shown to have higher fecal cortisol metabolites than F2 control offspring ( Schopper et al., 2012). Overall these studies suggest that prenatal stress may not only affect the exposed offspring, but may alter the phenotype of the following generations. This, in turn, suggests that prenatal stress may affect the disease risk in multiple generations. More research is needed to understand the mechanism underlying these trans-generational effects. From

a gene-environment mismatch theory perspective these trans-generational effects pose an interesting question. It seems that exposure to standard environmental conditions do not normalize the now FRAX597 mal-adaptive alterations in the F1 or F2 offspring. From an evolutionary standpoint, one may argue the absence of an environmental stressor in the current generation that was present in the previous generations may indicate variable environmental conditions, and since most of these mis-match pathologies develop after reproductive age, and thus will not diminish the population fitness, reversal of the phenotype has no priority. However, the “original” phenotype has to have some fitness advances otherwise this phenotype would have been lost during evolution. Thus one may inhibitors wonder which environmental cues would lead to “normalization” of the

phenotype, and whether we can mimic these environmental cues as a preventative strategy. Prenatal stress exposure alters the phenotype of the offspring, and when the postnatal environment does not match the prenatal environmental conditions these alterations may have pathological consequences. The studies discussed in this manuscript clearly indicate that there are some innate differences in Carnitine palmitoyltransferase II stress vulnerability, like the stress-coping style, that may impact an individuals’ risk of developing metabolic and other pathologies. Furthermore, this innate risk seems to be modulated by the prenatal environment, dependent on the genotype of the fetus, prenatal stress exposure may have adverse or protective properties. Additionally, to make risk prediction even more complex, the postnatal environment also interacts with both the genotype, and the prenatal environment. Using the stress-coping style model as an example, rats genetically selected for a passive stress-coping style have an increased risk to develop obesity.