It is worth selleckchem Volasertib pointing out that some tissue specific genes identified in leaf, cotyledon and root might be due to the infection of MNSV Ma5. Indeed, functional analysis indicated that leaf, cotyledon and root specific genes were enriched with GO terms such as response to stimulus and defense response. It is worth noting that one of the fruit specific genes encoded 1 aminocyclopropane 1 carboxylate oxidase, the final enzyme in the biosynthesis of ethylene which is a plant hormone that regulates ripening of cli macteric fruits. Further detailed digital expression analysis of this gene revealed that, as expected, the gene was predominantly expressed in fruits of melon cultivars Dulce and Vedrantais, both of which are climacteric fruits, while none or very few ACO transcripts were detected in fruits of the two non climacteric cultivars, PI161375 and Piel de Sapo T 111.

In addition, two genes encoding acyl carrier proteins were highly and exclusively expressed in fruit tissues. ACPs are essential components of the fatty acid synthase complex and may be required to maintain the production of fruit aroma volatiles. Interestingly, we found that genes involved in nucleo some and chromatin assembly and trans lation process were highly enriched in the list of flower specific genes. However, the exact role of these flower specific genes in melon flower development remains unclear and further studies are required to clarify their functions in flower development. Marker discovery from melon EST sequences Molecular markers are valuable resources for construct ing high density genetic maps, facilitating crop breeding and identifying traits of interest.

Early melon genetic maps mainly used markers of Restriction Fragment Length Polymorphism, Amplified Fragment Length Polymorphism, and Random Amplified Polymorphic DNA. However these types of mar kers are not user friendly as they are either labor inten sive to generate, harbor low rates of polymorphism in melon, or are not readily transferred to other genotypes and populations. With the accumulation of sequence information in melon during the past several years, markers of simple sequence repeats and sin gle nucleotide polymorphisms are becoming more widely used in construction of melon genetic maps.

These markers have the following advantages, they are hypervariable, multiallelic, codominant, locus specific, and evenly distributed throughout the genome, and for markers derived from ESTs, they are directly linked to expressed genes. The melon EST sequence informa tion generated in this and other studies has served as a major resource to generate new molecular markers. Several recently constructed melon high density genetic maps have already utilized SSR and SNP markers derived from EST sequences gen erated in the Carfilzomib present study.