Methods Strains This study included {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| 109 isolates of L. monocytogenes: 47 from human cases of listeriosis, 56 from different food products and food processing environments, and 6 from animals. Strains in this study were selected to include those associated with listeriosis outbreaks as well as sporadic cases and were representative of the serogroups most often associated with human disease. Forty nine isolates came from the UK-NRL: 35 were from UK clinical cases of listeriosis and 14 from foods and food processing environments isolated by UK-HPA Food Water and Microbiology Laboratories either
as part of routine food sampling or in response to listeriosis investigations. One of the UK isolates from a clinical case of listeriosis was included in the study as duplicate culture (Table 1). Table 1 PFGE and fAFLP discriminatory ability LBH589 using Listeria monocytogenes isolates of duplicate strains, associated with outbreaks or with sporadic cases Isolate Test Study (TS) group number[17] Responsible for sporadic (S) or outbreak (OB). Duplicate culture (D) Selleckchem Vistusertib Origin of isolate Country of origin Molecular serogroup1 PFGE 2 ApaI/AscI type fAFLP 2 HhaI/HindIII type 10CEB565LM n/a
OB 1 Human England IVb 326/136 IV4.3 10CEB567LM n/a OB 1 Food England IVb 326/136 IV4.3 10CEB550LM n/a OB 2 Human England IVb 178/6 I.8 10CEB552LM n/a OB 2 Food England IVb 178/6 I.8 10CEB553LM n/a OB 3 Human England IIa 149/109 III.10 10CEB554LM n/a OB 3 Food England IIa 149/109 III.10 10CEB559LM n/a OB 4 Human England IVb 309/142 UD4.1 10CEB560LM n/a OB 4 Food England IVb 309/142 UD4.1 10CEB542LM = 10CEB543LM3 n/a D Human England IIc 70/377 VIIc.8 TS32 02 S Food USA IVb 180/50 I.67 TS72 02 S Food USA IVb 180/50 I.67 TS56 = TS773 03 S4 and D Human USA IIa 120/191 VIIa.27 TS39 03 S Food USA IIa 120/191 VIIa.27a TS67 03 S4 Human USA IIa 120/191 VIIa.27a
TS17 05 S Human USA IIb 93/140 IVb.21 TS61 05 S Food USA IIb 93/140 IVb.21 TS31 15 OB 5 Human France IVb 24-Dec V.21 TS69 15 OB 5 Human France IVb 24-Dec V.21 TS21 16 OB 6 Food Switzerland IVb 19/15 V.3 TS55 16 OB 6 Human Switzerland IVb 19/15 V.3 Protirelin TS02 22 S25 Human England IIc 70/25 VIIc.1 TS08 22 S25 Human England IIc 70/25 VIIc.1 1 Serogrouping performed by multiplex PCR [4]: results are from both the European Reference Laboratory (EURL) for L. monocytogenes and the UK National Reference laboratory (UK-NRL) for Listeria. 2 PFGE was performed by the EURL and fAFLP by UK-NRL. 3 Serogrouping and typing results were the same for each of the duplicate culture. 4 The 2 patients of TS group number 3 were 2 separate sporadic cases and not epidemiologically linked [18]. 5 These 2 isolates are from the same patient who had 2 recurrent episodes of listeriosis [19]. n/a: not applicable.