No spots were observed 15 days post infection on the hepatopancreas surface.4. DiscussionResults strongly suggest that the American lobster can be a host for NHPB and that hepatopancreas malfunctions could occur during bacterial colonization.The thermal conditions at which lobsters Abiraterone structure were maintained during the experimental period (23��C) are near the upper limit that American lobster has been observed (27.5��C) [11]. Considering that the thermal preference of the American lobster is around 18��C [7], the conditions of the experiment could have affected the resistance of the lobster and favored the propagation of NHPB. However, evidence has demonstrated that the thermal limits of the Pacific white shrimp and the American lobster can be overlapped [7, 12]. According to the Sea Life Base (http://www.

sealifebase.org/), the distribution of the Pacific white shrimp has reached the East coast of the United States and apparently could be overlapped at some point with the distribution of the American lobster; however, a natural shrimp-lobster transmission has not been yet demonstrated.The results of the experiment are also evidence of the NHPB plasticity, because it can colonize different species from different thermal ecosystems. Hence, the hypothetic plasticity of NHPB could be the reason why it is yet detected in countries from different latitudes.Considering the above information, the worldwide distribution of white shrimp farms represents a risk for other crustacean species. White shrimp can be host not only for NHPB, but for a wide diversity of virus and bacteria [13].

Moreover, farms usually discharge effluents containing toxic metabolites and, in some cases, pathogenic microorganisms directly to the ecosystem [14, 15].On the other hand, considering that in vitro methods have not yet been developed for NHPB culture and that the hepatopancreas size from lobster can be 40- to 50-fold greater than those of adult white shrimp, the American lobster may be an adequate candidate to maintain available NHPB in vivo [8].In conclusion, the American lobster can be experimentally infected by NHPB, causing hepatopancreatic necrosis. This lobster can be a good experimental model to produce the bacteria in vivo. Results suggest the plasticity of NHPB to infect different crustacean species from different latitudes, but further studies are required to elucidate the infection process.
Cervical cancer is the second leading cause of cancer mortality in women in developing countries and seventh in developed countries Batimastat [1].