Thus, the light harvesting effect of dendrimers can be used to greatly Increase the sensitivity of the fluorescent sensors.\n\nThe progress described here demonstrates that highly enantioselective and sensitive fluorescent sensors can be obtained through a systematic investigation
of the structure-property relation between the sensors and the substrates. These I-BET-762 datasheet sensors show great potential for the development of rapid assays of chiral organic compounds.”
“Hydroxynitrile lyase (HNL) from seeds of Prunus pseudoarmeniaca was partially purified by (NH(4))(2)SO(4) fractionation and covalently immobilized onto Eupergit C and Eupergit C 250 L The percentages of bound protein per gram of Eupergit C and Eupergit C
250 L were about 81 and 98 of the initial amount of protein, respectively. K(m) and V(max) values were determined Selleck NVP-LDE225 2.23 mM and 0.54 U/mg prot. for the free HNL, 1.60 m M and 0.87 U/mg prot. for the immobilized HNL onto Eupergit C and 1.03 mM and 0.35 U/mg prot. for the immobilized HNL onto Eupergit C 250 L respectively at optimized reaction conditions. The half lives (t(1/2)) and the thermal inactivation rate constants (k(i)) of free and immobilized HNLs were determined at 25 and 50 degrees C, immobilized HNLs displayed higher thermal stability. Carboligation activities of free and immobilized HNLs for (R)-mandelonitrile (R-MN) synthesis were also determined. Besides, reusabilities of immobilized HNLs for both lyase and carboligation activities were investigated by using batch type buy FK506 reactors. (C) 2010 Elsevier B.V. All rights reserved.”
“Hepatitis B virus (HBV) has been classified into 8 genotypes (A-H). Genotypes A, D and F have been identified in some South American countries, but in Venezuela studies have been more restricted to aboriginal communities where genotype F is predominant. The aim of the present study was to identify the prevalence of HBV genotypes among native HBsAg carriers in
Venezuelan urban areas. In addition, we correlated the predominant HBV genotype with epidemiological, serological and virological features of the infection. Non-Venezuelan migrant patients were excluded from this study. Serum samples from 90 patients (21 children and 69 adults) with chronic hepatitis B (CHB) were analyzed. Seventy-four patients had CHB e-antigen positive and 16 CHB e-antigen negative. HBV DNA serum levels of the whole group ranged from 4.1 to 8.8 log(10) IU/mL. Patients with CHB e-antigen positive showed significantly higher viral loads (P = 0.0001) than the group with CHB e-antigen negative. Eighty-eight patients (97.8%) exhibited HBV genotype F while two non-related patients (2.2%) were infected with A + F genotypes. Genotype F is the main circulating HBV strain among HBsAg carriers from Venezuelan urban areas. This genotype is associated mostly with CHB e-antigen positive and high rate of transmission.