We aimed at comparing ruthenium (Ru-106, emitting beta electrons) and iodine (I-125, gamma-radiation) brachytherapy and proton beam therapy of melanoma implanted into the hamster eye on development of spontaneous lung metastases. Tumors of Bomirski Hamster Melanoma (BHM) implanted into the anterior chamber of the hamster eye grew aggressively and completely filled the anterior Ivacaftor structure chamber within 8-10 days. Metastases, mainly in the lung, were found in 100% of untreated animals 30 days after enucleation. Tumors were irradiated at a dose of 3-10 Gy with a Ru-106 plaque and at a dose of 6-14 Gy using a I-125 plaque. The protons were accelerated using the AIC-144 isochronous cyclotron operating at 60 MeV. BHM tumors located in the anterior chamber of the eye were irradiated with 10 Gy, for the depth of 3.
88 mm. All radiation types caused inhibition of tumor growth by about 10 days. An increase in the number of metastases was observed for 3 Gy of beta-irradiation, whereas at 10 Gy an inhibition of metastasis was found. gamma-radiation reduced the metastatic mass at all applied Inhibitors,Modulators,Libraries doses, and proton beam therapy at 10 Gy also inhibited the metastastic spread. These results are discussed in the context of recent clinical and molecular data on radiation effects on metastasis.
Cancer chemotherapy is associated with serious side effects, including temporary hair loss and impairment of pigmentation. We suspect that ectopic melanin deposition occurring due to chemotherapy may add to these effects worsening the already unpleasant symptoms.
We associated the ectopic occurrence of follicular melanin after chemotherapy with splenic melanosis an interesting example Inhibitors,Modulators,Libraries of extradermal melanin localization and we expected an increase in splenic melanin deposition after chemotherapy. Using the C57BL/6 murine model of synchronized hair cycle induced by depilation, we visualized splenic melanin by means of several histological and histochemical protocols of staining: hematoxylin and eosin, May-Grunwald-Giemsa and Fontana-Masson. Inhibitors,Modulators,Libraries Unexpectedly, the splenic deposition of melanin decreased due to application of cyclophosphamide (i.p. 120 mg/kg body weight on day 9 post depilation). The drop was abrupt and lasted for at least 5 days (day 13-18 post depilation), as compared with normal hair cycle.
Moreover, in mice with normal, depilation-induced hair cycle we observed a similar drop shortly before entering catagen (day 15 post depilation), followed by a slow and partial increase in splenic melanization up to day Inhibitors,Modulators,Libraries 27 post depilation in both groups. We conclude that cyclophosphamide negatively affects splenic melanization and/or extradermal transfer Inhibitors,Modulators,Libraries of ectopic melanin from the dystrophic hair follicles, but the most powerful down-regulator of splenic melanosis is normal and dystrophic catagen the phase of hair follicle involution and re-modelling.
Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, selleck chemical are caused by metastases.