We found that amphetamine had no effect on Wnt5a or Wnt7a expression but increased Wnt3. We then measured protein expression of Wnt3, phosphorylated lipoprotein-related peptide 6, GSK-3 beta phosphorylated at serine-9 and tyrosine-216 and total beta-catenin. We found that amphetamine increased Wnt3
protein expression, increased pLRP6 (threonine-1572) levels, increased beta-catenin levels, increased GSK-3 beta phosphorylation at serine-9, consistent with inhibition of GSK-3 beta activity, and diminished GSK-3 beta phosphorylation at tyrosine-216. Our data support the hypothesis BMS-754807 concentration that proximate Wnt signalling is rapidly activated by amphetamine in the adult rat nucleus accumbens. NeuroReport 23:846-850 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.”
“The primary goal of this study was to examine the biochemical abnormalities of late-life major depression by using 3-tesla (3-T) proton magnetic resonance spectroscopy ((1)H-MRS). The antidepressant effects on the biochemical abnormalities were investigated as well. Study participants were 27 elderly patients with major depressive disorders (among which 9 were
on antidepressant medication) and 19 comparison elderly subjects. (1)H-MRS spectra were acquired from voxels that were placed in the left frontal white matter, left periventricular LY294002 purchase white matter, and left basal ganglia. Ratios of N-acetylaspartate (NAA), choline (Cho) and myo-inositol to creatine were calculated. Patients with late-life major depressive disorder had a significantly lower NAA/creatine ratio in the left frontal white Magnesium chelatase matter, and higher Cho/creatine and myoinositol/creatine ratios in the left basal ganglia when compared with the control subjects. The myoinositol correlated with global cognitive function among the patients. The biochemical abnormalities in late-life major depressive disorder
were found on the left side of the frontal white matter and the basal ganglia. Neuron degeneration in the frontal white matter and second messenger system dysfunction or glial dysfunction in the basal ganglia are suggested to be associated with late-life depression. (C) 2009 Elsevier Ireland Ltd. All rights reserved.”
“The association of genetic lesions detected by fluorescence in situ hybridization (FISH) with survival was analyzed in 1069 patients with newly presenting myeloma treated in the Medical Research Council Myeloma IX trial, with the aim of identifying patients associated with the worst prognosis. A comprehensive FISH panel was performed, and the lesions associated with short progression-free survival and overall survival (OS) in multivariate analysis were +1q21, del(17p13) and an adverse immunoglobulin heavy chain gene (IGH) translocation group incorporating t(4;14), t(14;16) and t(14;20).