coli O157, K. pneumonia, P. mirabilis, and E. sakazakii on the standard medium Discussion Plasma treatment is considered a good and safe method to eliminate the decontamination of not only dental instruments but also general surgical instruments.10 Our results showed that the best bacterial inactivation plasma conditions were 300 W applied power, 4.5 cm distance from the

source, and 1.24 mbar pressure at 9% of O2. Philip et al.11 demonstrated that total inactivation of Bacillus subtilis spores was achieved 40 minutes after plasma exposure at 100 W with 2% of O2. Furthermore, Xu et Inhibitors,research,lifescience,medical al.1 reported that the time needed for the inactivation of Geobacillus stearothermophilus spores was 3 minutes. In another Inhibitors,research,lifescience,medical study, Xu et al.1

also found that 10-20% of O2 was sufficient to inactivate these bacteria. Elsewhere, Feichtinger et al.12 discovered that spores numbers were reduced one second after the application of laboratory air as plasma gas. Our results agree with those reported by Xu et al.,13 who revealed that using argon (Ar) in a plasma jet source for 10 minutes did not totally eliminate E. coli. According to our results, O2-N2 gas using a plasma source Inhibitors,research,lifescience,medical was able to totally inactivate all kinds of bacteria except E. coli. The inactivation effect was more pronounced when we used flat polymers as substrates. Ricard and Monna14 reported that N2–5% O2 gas mixture completely eliminated Inhibitors,research,lifescience,medical Streptococcus H 89 datasheet mutans, Porphyromonas gingivalis, and Prevotella intermedia bacteria 15–20 minutes after treatment. In contrast, our results demonstrated that SF6 gas totally inactivated the bacteria in only 1-3 minutes. Conclusion Plasma inactivation using N2-O2 gas mixture and SF6 gas proved promising for the inactivation of the bacterial isolates in the Inhibitors,research,lifescience,medical present study. Our findings could be helpful in many medical and industrial fields; however, further investigations are needed to integrate this technique into the field of bacteria disinfection.

Acknowledgment The authors would like to thank the Director General of AECS, enough the Head of the Physics Department, the Head of the Chemistry Department, and the Head of the Molecular Biology and Biotechnology Department for their support. Conflict of interest: None declared.
Background: Coronary angiography consists of the selective injection of contrast agents in coronary arteries. Optimal strategy for heparin administration during coronary angiography has yet to be determined. We assessed the effect of heparin administration during coronary angiography on vascular, hemorrhagic, and ischemic complications. Methods: Five hundred candiates for diagnostic coronary angiography (femoral approach) were randomly divided into case (intravenous Heparin [2000-3000 units]) and control (placebo) groups.