First, one can suggest that this allele has been inactivated or importantly down regulated in the CI-inducing strains of isopods. Change in regulatory element repertoire and divergence in patterns of expression
may occur after small-scale duplication of the genome [36]. A corollary to a change in location, paralogous and homologous pk2 copies within and among Wolbachia strains would have followed different evolutionary trajectories leading to such a phenotypic diversity. Second, genomic imprinting, process by which genes are expressed from only one parental allele due to epigenetic mechanism, can be considered as a molecular mechanism underlying the diversity of phenotypes. Recently, early changes in gene imprinting and aberrant expression of specific genes have
been shown to be coupled to parthenogenesis in mice embryos [37]. Third, one AC220 datasheet can suggest that genes in the pk2 family could have diverse functions. In this way, BIX 1294 post-transcriptional modifications and dosage of Wolbachia products, as well as genetic control by the host, cannot be dismissed. As previously suggested [38], Selleckchem FHPI differences in Wolbachia-induced feminization as well as the presence of the bacteria in O. asellus males, may simply result from differences in bacterial dosage or in host targets. The basic molecular mechanisms that mediate Wolbachia feminization are also still unknown
although it is unlikely that this effect is driven by only one gene. In A. vulgare Wolbachia effectors may target the proteinaceous androgenic hormone or its receptor, or another major sex determinant, thereby inhibiting the androgenic gland differentiation and preventing the androgenic hormone from reaching the target tissues such as gonads and tegumental epithelium [2, 39, 40]. This hypothesis suggests a late action of feminizing Wolbachia on host target(s) during its development, as opposed to the very early action of other Wolbachia strains that induce parthenogenesis, CI or male killing [5, 41]. Conclusions Our results highlight a large copy number variation of both pk1 and pk2 genes among strains, likely Tolmetin linked to prophage diversity, and also the specific expression of one pk2 allele only in the feminizing Wolbachia strains of isopods. This correlation supports the hypothesis that phenotype-related effectors or specific strain determinants in Wolbachia are likely to be encoded by prophage genes, ankyrin-repeat encoding genes, and predicted genes of unknown function [42]. Our results thus reveal the need to search for host molecules targeted by Wolbachia ankyrins and their functions with respect to host sex manipulation by Wolbachia. Methods Wolbachia-infected isopod species All isopods used in this study were collected in France and reared in the laboratory.