Table 7 Response on climate change

regarding flight behav

Table 7 Response on climate change

regarding flight behaviour and mobility Type of flight behaviour/mobility per species C. pamphilus M. jurtina M. athalia P. argus Duration of flying bouts + + + + Tendency to start flying + + + = Proportion of time spent flying + – + = Tortuosity = = = = Net displacement + – + = +, increase; −, decrease; =, neutral The possibility to reach new habitats is a prerequisite under changing climatic conditions (Vos et al. 2008). Individuals must be able to cross distances over unsuitable environments. This study indicates that climate change may increase dispersal propensity in butterflies, as ectothermic species with LCZ696 manufacturer generally poor mobility. Incorporation of these insights in metapopulation MK5108 datasheet models

is necessary to improve predictions on the effects of climate change on shifting ranges. Acknowledgments This research was funded by the Dutch national research programme ‘Climate Changes Spatial Planning’ and is part of the strategic research programme ‘Sustainable spatial development of ecosystems, landscapes, seas and regions’ (Project Ecological Resilience) which is funded by the Dutch Ministry of Agriculture, Nature Conservation and Food Quality, and carried out by Wageningen University and Research Centre. The Dutch Butterfly Monitoring Scheme is a joint project by Dutch Butterfly Conservation and Statistics Netherlands (CBS), supported financially by the Dutch Ministry of Agriculture, Nature and Food Quality. We thank Paul Opdam for helpful comments on the manuscript; the staff of the National Park “De Hoge Veluwe” for permission to work in the Park; Larissa Conradt, René Jochem, Dynein Ruut Wegman, and members of the “Friends of the Hoge Veluwe” Fauna working group for practical

help and tips on the fieldwork; and Gerrit Gort and Hans Baveco for help on statistics. Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. Appendix 1 See Fig. 4. Fig. 4 Kaplan–Meier survival curve for flying bouts of M. athalia with temperature as single covariate. Under low temperature (solid line; less or equal to 14°C), butterflies terminate flying bouts sooner than under intermediate temperature (between 14 and 25°C; dashed line; P = 2.9E − 08) and high temperature (more than 25°C; dotted line; P = 1.1E − 09). Appendix 2 See Table 8. Table 8 Correlations between covariates from field study   Species C. pamphilus G Y T R C W Gender (G) 1           Year (Y) 0.30 1         Temperature (T) 0.03 −0.42 1       BTSA1 concentration Radiation (R) −0.05 −0.23 0.44 1     Cloudiness (C) −0.09 0.31 −0.67 −0.30 1   Wind speed (W) −0.06 −0.07 0.05 0.33 −0.13 1   Species M. jurtina G Y T R C W Gender (G) 1           Year (Y) 0.33 1         Temperature (T) −0.21 −0.84 1       Radiation (R) 0.15 0.20 −0.

A phylogenetic tree was constructed to investigate the evolutiona

A phylogenetic tree was constructed to investigate the evolutionary relationships between these proteins. Based on the sequence divergence in amino acid TyrDC sequences (Figure 1), the phylogenetic tree reveals that L. FHPI plantarum TyrDC is closely related to those of L. brevis proteins and made one cluster clearly separated. Similar results were

obtained when phylogenetic tree was constructed with TyrP amino acid sequences (data not shown). These results confirm that the organization of this L. plantarum tdc MEK activation locus is similar to those described for other LAB strains, with contiguous tyrDC and tyrP genes. The phylogenetic tree analysis is consistent with the tdc locus of L. plantarum IR BL0076 strain having been transferred horizontally from L. brevis. Figure 1 Phylogenetic tree comparing 21 TyrDC sequences from various Lactobacillus strains. The amino acid sequences were aligned using the multiple alignment program

CLUSTAL W2. The phylogenetic tree was constructed by using the TreeTop from the GeneBee. Bootstrap values are expressed in percentages and indicated at nodes. The amino acid sequences of TyrDC were obtained from the following accession numbers entries: [GenBank : AF446085] (L. brevis IOEB 9809), [GenBank : YP_796294.1] (L. brevis ATCC 367), [GenBank : ABY71221.1] (L. brevis NS77), [GenBank : ZP_03940842.1] (L. brevis subsp. gravesensis ATCC 27305), [GenBank :AEB91325.1] (Sporolactobacillus sp. P3J), [GenBank

: AAQ73505.1] ICG-001 molecular weight (E. hirae), [GenBank : ZP_05553037] (L. coleohominis 101-4-CHN), [GenBank : ZP_07729457] (L. oris PBo13-T2-3), [GenBank :ZP_06679761] (E. faecium E1071), [GenBank : ZP_06677337] (E. faecium E1162), [GenBank : ZP_00602894.1] (E. faecium DO), [GenBank : ZP_06698865.1] (E. faecium E1679), [GenBank : CAF33980] (E. durans IPLA 655), [GenBank : ZP_05559869] (E. faecalis T8), [GenBank : ZP_07768147] (E. faecalis DAPTO 516), [GenBank : ZP_07771864] (E. faecalis TX0102), [GenBank : ZP_07569615] (E. faecalis TX0109), [GenBank : CBL32775] (Enterococcus sp. 7 L76), [GenBank : ADX79254] (E. faecalis 62) and [GenBank : ZP_04646316] (E. faecalis TUSoD Ef11). Growth of L. plantarum with peptides containing tyrosine Peptides of different sizes were used: Non-specific serine/threonine protein kinase a dipeptide Tyr-Ala containing the tyrosine residue at the N-terminus, a tripeptide Gly-Leu-Tyr with the tyrosine at the C-terminus, and a peptide of four amino acids Gly-Gly-Tyr-Arg, where the tyrosine is in an internal position. The growth was monitored by measuring the OD at 600 nm. L. plantarum IR BL0076 was able to grow in the synthetic medium either with free amino acids (medium 1) or synthetic peptides containing tyrosine (medium 2). The growth curve was the same in the two media (Figure 2), but not in MRS medium (control).

Experimental procedures In order to evaluate the blood leukocyte

Experimental procedures In order to evaluate the blood leukocyte and glucose levels of C. callosus infected with P. brasiliensis, the animals were i.p. injected followed by macroscopic and microscopic evaluations done at days 7, 15, 30, 45, 60, and 75 post infection (three to four animals were analyzed

per group at each time point of infection). The organs showing macroscopic lesions were selected for further analysis. Control groups consisted of three animals per time point C59 wnt research buy inoculated with sterile saline. To determine the role of estrogen during P. brasiliensis infection, an additional C. callosus group (seventy animals) was subdivided into two sets: one being bilaterally click here ovarectomized (31 animals) and the other sham-operated (39 animals). Forty days after surgery, all animals were inoculated in the peritoneum with 1 × 106 viable infective forms of P. brasiliensis. An additional control group consisting of non-operated and non-infected animals (5 animals per

time point) received only saline injection. Histology On days 15, 45, 60, and 75 of infection, two to three animals from each group were sacrificed, grossly inspected, and fragments of mesentery, liver, spleen, pancreas, and lungs were collected and fixed in 10% formaldehyde. Representative sections from each organ were embedded in paraffin, processed and stained with haematoxilin-eosin (HE). Quantification of the lesion extensions was determined using a computer-aided densitometric software (OPTIMAS Bioscan Inc. WA, acetylcholine USA). For each organ, five slides with tissue sections were entirely evaluated. The number and area of the granulomas were determined, and the extent of tissue section occupied by the lesion was calculated by dividing the area occupied with lesions by the total area of the organ. Leukocyte counts and glucose levels Blood samples for leukocyte counts or glucose determinations were withdrawn from the retro-orbital plexus. Leucocytes were counted in a haemocytometer and the results were reported

as number of leukocytes per mL of blood. Serum glucose levels were determined by the method of Trinder [18] and reported as mg/dL. Results PB01 infection in Calomys callosus Gross inspection of C. callosus i.p. infected with 106 yeast forms of PB01 revealed peritonitis characterized by the presence of exudates containing a large number of yeast cells. Adherence involving several parts of mesentery and spleen was also observed. These signs increased in intensity with time from injection of the fungus until the infection turned to the chronic phase (sixty days post infection). Following the acute phase of the inflammatory reaction, the infection became circumscribed due to granuloma formation in the peritoneal cavity as well as in several distant organs such as the liver, spleen, lungs, and pancreas.

An exacerbation of COPD caused by H influenzae was defined by th

An exacerbation of COPD caused by H. influenzae was defined by the onset of clinical symptoms of an exacerbation simultaneous with the CH5183284 clinical trial acquisition of a new strain of H. influenzae that had not previously been isolated from that

patient based on molecular typing [54]. Proteasome inhibitor Serum samples collected one month prior to acquisition of the strain and one month following the exacerbation were used to analyze human serum antibody responses to the purified recombinant urease C. Pooled human sputum Expectorated sputum samples were collected from subjects in the COPD Study Clinic and were processed for culture as previously described [54, 62]. Briefly, sputum samples were homogenized by incubation at 37°C for 15 minutes with an equal volume of 0.1% dithiothreitol. After an aliquot was removed for quantitative culture, sputum samples were centrifuged at 27,000 × g for 30 minutes at 4°C and supernatants were stored at -80°C until used. Samples from patients who were receiving antibiotics and samples that grew potential pulmonary bacterial pathogens in culture were excluded. find more Supernatants from

approximately 100 sputum samples from 30 individuals were pooled for the purpose of growing bacteria in pooled sputum supernatants [13]. To render the sputum supernatants sterile, the pooled samples were placed in Petri dishes and exposed to UV light in a cell culture hood for approximately 10 minutes. An aliquot was plated on chocolate agar and no growth was detected after overnight incubation. Quantitative real time PCR H. influenzae was grown in the presence pooled human sputum from adults with COPD to simulate conditions in the human respiratory much tract. To assess transcription of ureC, strain

11P6H was grown overnight in chemically defined media (CDM) at 37°C with shaking to which pooled human sputum supernatant of 20% of the volume of the culture was added [13]. A second culture was grown simultaneously in CDM to which PBS containing 0.1% dithiothreitol was added to 20% of the total volume as a control for the sputum supernatant. Cells were harvested by centrifugation at 10,000 × g for 10 minutes at 4°C. Cells were washed by suspending in cold PBS and centrifuging again using the same conditions. Bacterial RNA was isolated as described above (Reverse Transcriptase-PCR). Quantitative real time PCR was performed using the BioRad MyiQ Real-Time PCR Detection System. Oligonucleotide primers pairs (Table 2) were designed with Primer 3 software. Each reaction mixture contained 5 ng purified RNA, 100 nM of each primer, 12.5 μl 2 × Sybr Green Supermix (BioRad), 0.125 μl reverse transcriptase and 6.375 μl water. Controls lacking reverse transcriptase or RNA template contained the appropriate volume of water in place of enzyme or template. Each purified RNA sample was tested for DNA contamination prior to proceeding with the real time PCR assay.

Results shown are representative of three separate experiments E

Results shown are representative of three separate experiments. Expression of IL-8 mRNA was quantified by densitometry, and standardized by the β-actin level. *p < 0.05, **p < 0.01 compared with the level at 1 h or 2 h. PMA: phorbol 12-myristate 13-acetate. Induction of IL-8 release by PCN learn more in PMA-differentiated U937 cells Previous studies have identified that PCN stimulates IL-8 production by lung macrophage cells [23] and surface epithelial cells [8, 14, 24]. Based on the physical properties of PCN, we hypothesized that

it was able to stimulate differentiated U937 cells to produce IL-8. To test this hypothesis, we exposed differentiated human U937 cells to purified PCN and measured its effects on the release of IL-8. After 24 hours Selleck CUDC-907 of incubation with different concentrations of PCN (5 μM, 25 μM, or 50 μM) in PMA-differentiated U937cells, the supernatants were collected and IL-8 release detected by ELISA. The results showed that PCN increased IL-8 release in differentiated U937 cells in a concentration-dependent manner. An increase in IL-8 release was observed with PCN concentration at as low as 5 μM and the concentration of 50 μM produced the strongest stimulation as to the cellular response (Figure 2A and B). The increase in

IL-8 above SGC-CBP30 cost control levels was observed at as early as 8 h after PCN (50 μM) addition, and these levels continued to increase between 24 h and 48 h (data not shown). Longer periods of incubation were not tested. Figure 2 PCN increases IL-8 release in PMA-differentiated U937 cells. (A) Different concentrations of PCN (5 μM, 25 μM, or 50 μM) were added to the cell cultures for 24 h. Supernatants were harvested for measuring IL-8 secretion by ELISA. (B) A fixed concentration of PCN (50 μM) was added to the cell cultures PAK5 for 8, 16 or 24 h. Supernatants

were harvested for measuring IL-8 level by ELISA. Values represented are the mean ± SD of four independent experiments in triplicate. **p < 0.01 compared with PMA-differentiated U937 cells. PMA: phorbol 12-myristate 13-acetate. The oxidative effect of PCN on differentiated U937 cells A previous study has shown that PCN induces a concentration-dependent loss of cellular glutathione (GSH), an important cellular antioxidant, up to 50% in the tissues infected by P. aeruginosa [25]. N-acetyl cysteine (NAC) is the precursor of GSH. So we hypothesized that NAC may play a protective role in cells exposed to PCN. Thus, different concentrations of PCN (5, 25, and 50 μM) were added into differentiated U937 cells, and the supernatants were collected after 24 hours. We then detected the leakage of LDH, the content of MDA, and the activities of SOD and CAT using their respective detection kits.

In that trial, cats were randomized to receive bleomycin ± the im

In that trial, cats were randomized to receive bleomycin ± the implant of 30 × 106 CHO cells (secreting interleukin 2) followed by the application of square pulses. The study was completed by a small cohort of untreated cats that acted as control. The authors described only one partial response however, they claimed a prolonged survival in 12 cats receiving ECT versus 11 untreated controls. This minimal response rate could be partially

due to the previous treatments that led to the development of chemoresistance. In fact, it is known that radioresistant neoplasms have increased DNA repair which is one of the described mechanisms of resistance to bleomycin as well, at least in cell lines learn more [15]. After this preliminary investigation, two phase I/II studies were conducted in companion animals; in the first a cohort of dogs and cats were treated with intralesional cisplatin coupled with square electric pulses [23] while in the second they received intralesional bleomycin driven by trains of biphasic pulses [19]. The overall response rate of this

second investigation was 80% with a 40% of long lasting remissions. This study evidenced that among the treated neoplasms, canine hemangiopericytomas were particularly responsive to this approach. This work Blasticidin S ic50 evidenced two problems of ECT: the need of specifically tailored electrodes for the therapy of soft tissue neoplasms and the major obstacle to a smooth permeabilization represented by the high content of connective tissue within solid tumors [24]. Currently, ECT is preferentially adopted as single modality only for tumors very susceptible to electroporation such as melanomas and perianal adenomas [34–36] or relatively small in size and easily accessible like sun-induced nasal carcinomas [29]. In selected patients with cutaneous epitheliotropic and non-epitheliotropic lymphoma this therapy can lead to successful palliation or even extended local control and, consequently, survival [37]. After the development of novel electrodes [25], several phase II studies were conducted in our

Institution to evaluate the potential of ECT as adjuvant treatment after surgical cytoreduction of bulky tumors mimicking the protocols of intraoperative radiation therapy [38]. A preclinical study involving cats with soft tissue sarcomas, Glutamate dehydrogenase evaluated the potentials of intraoperative and check details postoperative ECT [26]. Cats were randomized to the following groups: surgery single modality, surgery plus intraoperative ECT and surgery plus postoperative ECT. The study underlined the significant advantage offered by adjuvant ECT in terms of local control and overall survival compared to surgery alone. Time to recurrence was 12 and 19 months for the intraoperative and postoperative cohorts respectively, while the tumors treated with surgery alone recurred within an average of 4 months.

oneidensis Fur regulates genes involved in iron homeostasis and a

oneidensis Fur regulates genes AZD8931 supplier involved in iron homeostasis and acid resistance [10–13]. Consistently, many of these target genes have a recognizable “”Fur box”" in their promoters. In the present study, we further characterize a fur null mutant of S. oneidensis with regard to its ability to utilize succinate and fumarate. Unexpectedly,

HPLC analysis showed that the fur mutant was able to metabolize succinate and fumarate, and the growth of the mutant was enhanced in the presence of succinate and fumarate, indicating that the mutant can utilize these compounds. In addition, the expression of the TCA cycle genes acnA and sdhA was not down-regulated in the Selleck GW3965 mutant. These differences between S. oneidensis and E. coli were traced to the small RNA gene ryhB, which we identified Selleckchem Barasertib in several Shewanella species. Although S. oneidensis RyhB was up-regulated in the fur mutant, the TCA cycle genes did not appear to be regulated by RyhB. These results delineate differences in the gene regulation and physiological consequences of RyhB between S. oneidensis and E. coli. Results TCA cycle activity and regulation in the fur mutant We showed recently that S. oneidensis harboring a fur deletion in the genome was sensitive to acidic conditions and de-repressed genes encoding iron acquisition systems [11]. Similar observations

have been made in E. coli [14, 15], suggesting that the functional roles of Fur are conserved in these species. Since Fur acts as a pleiotropic transcription factor involved in multiple biological processes, we proceeded to examine its role in regulating TCA cycle enzymes. The involvement of Fur in this biological process has been established in E. coli and V. cholerae by observations that fur mutants are unable to grow in defined

media with succinate or fumarate as a carbon source [9, 16], and that genes encoding certain TCA cycle enzymes, such as succinate dehydrogenase (SdhABCD) and aconitase (AcnA), are significantly down-regulated in a fur mutant [7]. Our initial tests showed that neither succinate nor fumarate, when provided as the sole carbon source in M1 defined media, could support detectable growth of S. oneidensis type strain MR-1 (data not shown), making it unlikely to Morin Hydrate analyze the growth of MR-1 and fur null mutant. However, the complete set of TCA genes is present in S. oneidensis genome, and recent studies have shown that the bacterium is capable of metabolizing succinate and fumarate [17, 18]. To compare the metabolizing rates of the carbonates between MR-1 and the fur mutant, both strains were grown to mid-log phase with 10 mM lactate as the carbon source. Then equal numbers of cells (5 × 109) were washed and resuspended in fresh M1 medium with 10 mM lactate, succinate or fumarate as the sole carbon source.

However, the use of organic media and the synthesis of polydisper

However, the use of organic media and the synthesis of polydisperse nanoparticles limit their use for some specific applications in where monodisperse nanoparticles are required [24, 25]. Alternative procedures for the synthesis of Au or AgNPs are BIBF 1120 based on the use of water soluble polymers with the aim of achieving size-controlled nanoparticles. Wang and co-workers have obtained AuNPs in aqueous solution in the 1–5 nm size range with the use of poly(methacrylic acid) (PMMA) [26, 27]. Keuker-Baumann

and co-workers reported a study about the formation of AgNPs with a high control and a characteristic plasmon band at 410 nm is observed using dilute solutions of long-chain sodium polyacrylates (NaPA) by exposing the solutions to UV-radiation [28] in where the coil size of the polymeric selleck compound chains acts as a collector of silver cations (Ag+). Other researches have investigated the formation of AgNPs and intermediate

clusters in polyacrylate aqueous solutions by chemical reduction of Ag + using a reducing agent, gamma radiation or ambient light [29–32]. Very recently, our group has described the synthesis of multicolor silver nanoparticles with a high stability in time, using poly(acrylic acid, sodium salt) (PAA) as a protective agent, in where the AgNPs exhibit localized surface plasmon resonance (LSPR) spectra (colors) as a function of variable protective and reducing agents with a well-defined shape and size [33]. Once triclocarban the metallic nanoparticles have been synthesized, a further assembly in the form of thin films is required to obtain the desired silver nanoparticle composites. However, this is not always possible Erismodegib concentration because of the need of preserving the

aggregation state of the nanoparticles. Several approaches are based on the incorporation of the nanoparticles into a previous polymeric matrix obtained by different thin film techniques, such as sol–gel deposition or electrospinning process [34, 35]. In all the cases, the presence of an intense absorption band at 410 nm is indicative of spherical AgNPs with a characteristic yellow coloration. In this work, layer-by-layer (LbL) assembly allows to manipulate and incorporate the nanoparticles into the thin films due to the use of PAA as a protective agent which maintains unaltered the aggregation state of the AgNPs. This technique is based on the alternating deposition of oppositely charged polyelectrolytes in water solution (polycations and polyanions) on substrates where the electrostatic interaction between these two components of different charge is the driving force for the multilayer assembly [36]. Previous works are based on the in situ synthesis of AgNPs in the polyelectrolyte multilayers via counterion exchange and posterior reduction [37–41].

Gupta PB, Onder TT, Jiang G, Tao K, Kuperwasser C, Weinberg RA: I

Gupta PB, Onder TT, Jiang G, Tao K, Kuperwasser C, Weinberg RA: Identification of selective inhibitors of cancer stem cells by high-throughput screening. Cell 2009, 138:645–659.PubMedCrossRef 41. Li L, Yu H, Wang X, Zeng J, Li D, Lu J, et al.:

Expression of seven stem-cell-associated markers in human airway biopsy specimens obtained via fiberoptic bronchoscopy. J Exp Clin Cancer Res 2013, 32:28.PubMedCrossRef 42. Chen Z, Wang T, Cai L, Su C, Zhong B, Lei Y, et al.: Clinicopathological significance of non-small cell lung cancer with high prevalence of Oct-4 tumor cells. TSA HDAC mw J Exp Clin Cancer Res 2012, 31:10.PubMedCrossRef 43. Wang Q, Mora-Jensen H, Weniger MA, GW-572016 mouse Perez-Galan P, Wolford C, Hai T, et al.: ERAD inhibitors integrate ER stress with an epigenetic mechanism to activate BH3-only protein NOXA PF-3084014 molecular weight in cancer cells. Proc Natl Acad

Sci USA 2009, 106:2200–2205.PubMedCrossRef 44. Hayashi T, Saito A, Okuno S, Ferrand-Drake M, Dodd RL, Nishi T, et al.: Oxidative damage to the endoplasmic reticulum is implicated in ischemic neuronal cell death. J Cereb Blood Flow Metab 2003, 23:1117–1128.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions LS and XL designed research; XZ and LS performed research; XZ and LS analyzed data; XZ, XL and LS wrote the paper. All authors read and approved the final manuscript.”
“Background Gastric cancer is one of the most prevalent malignant tumors, especially in Asia [1]. Although early detection methods, development of endoscopic or surgical resection, and more effective chemotherapies have improved the overall survival in patients with gastric cancer, the prognosis of patients with advanced gastric cancer is still poor [2–4]. Most conventional chemotherapy treatments have demonstrated

moderate efficiency. One possible explanation Sirolimus for the resistance of gastric cancer to conventional therapy might be its non-susceptibility to apoptosis [5]. However, oncolytic viruses have great therapeutic effects against cancer cells which express high levels of ribonucleotide reductase, DNA-repair enzymes, and are thus resistant to apoptosis [6, 7]. Many of these characteristics which make gastric cancer cells resistant to chemotherapy, make them susceptible to oncolytic viral therapy. Thus, gene therapy using oncolytic virus offers an attractive alternative for the treatment of gastric cancer [8]. Oncolytic viral therapy has been studied over the past century and shown success in preclinical and clinical testing as a novel cancer treatment modality [9]. Vaccinia virus (VACV) strains are particularly attractive as potential antitumor agents, as they can incorporate large amounts of foreign DNA without reducing their replication efficiency. Moreover, VACV has shown a great safety profile in humans [10–12].

24 N, 58 39 W + AMNH; McDiarmid, (1973) Kangaruma 05 18 N, 59 17 

24 N, 58.39 W + AMNH; McDiarmid, (1973) Kangaruma 05.18 N, 59.17 W + AMNH; McDiarmid (1973) Karisparu 04.58 N, 59.30 W + BM Kartabo 06.21 N, 57.50 W + AMNH; McDiarmid (1973) Potaro River 05.20 N, 59.17 W + BM 25 mi WSW of Mabura Hill* 05.13 N, 59.21 W + AMNH Peru (31 localities, 21 presences) Achinamisa, Depto. San Martín 06.25 S, 75.54 W + AMNH Balta, Depto. Ucayali 10.08 S, 71.13 W − Duellman and Thomas (1996) CB-839 datasheet Barranca,

Depto. San Martín 07.16 S, 76.28 W + AMNH Bolognesi selleck region, Depto. Ucayali 10.02 S, 73.57 W − Lehr (2002) Cachiyacu, Depto. San Martín 05.44 S, 77.29 W + Rivero (1968) Chayahuitas, Depto. Loreto 05.50 S, 76.10 W + Rivero (1968); Lötters et al. (2002) Cocha Cashu/PN Manu, Depto. Madre de Dios 11.54 S, 71.22 W − Rodríguez (1992) Cuzco Amazónico, Madre de Dios 12.35 S, 69.05 W − Duellman and Salas (1991) Explorama, Depto. Loreto 02.35 S, 71.57 W − Duellman and Thomas (1996) Genaro Herrera, Depto. Loreto 04.59 S, 73.46 W + MUSM Iquitos region, Depto. Loreto* 03.40 S, 73.20 W + AMNH; Rodríguez and Duellman (1994) Manseriche, Depto. Loreto 04.25 S, 77.35 W + Rivero (1968) Milagros, Depto. Ucayali 10.08 S, 74.01 W − Lehr (2002) Monte this website Alegre, Depto. Loreto 06.42 S, 74.15 W + AMNH Nauta region, Depto. Loreto 04.30 S, 73.40 W + Asquith and Altig (1987) Panguana, Depto. Huánuco 09.35 S, 74.48 W − Schlüter

(2005) Pebas region, Depto. Loreto 03.20 S, 71.50 W + AMNH; Lescure (1981a) Roabaya, Depto. Loreto 04.10 S, 73.20 W + Rivero (1968) Río Ampiyacu, Depto. Loreto 03.10 S, 72.00 W + Lötters et al. (2002) Río Cachiyacu, Depto. Loreto 08.09 S, 76.32 W + Lötters et al. (2002) Río Loretoyacu, Depto. Loreto 03.49 S, 70.26 W + AMNH Río Pisqui, Depto. Loreto 08.05 S, 75.35 W + Lötters et al. (2002) Río Sepahua, Depto. Ucayali 11.10 S, 73.01 W + Rivero (1968) Río Távara, Depto. Puno* 13.31 S, 69.41 W + Bärtschi and MacQuarrie (2001) Río Tambo, Depto. Loreto 01.15 S, 75.21 W + Rivero (1968) Galeterone Río Yubineto, Depto. Loreto 01.02 S, 74.13 W + Lescure and Gasc (1986), Lescure (1981a) San Jacinto, Depto. Loreto 02.19 S, 75.52 W − Duellman and Mendelson (1995) Tacsha, Depto. Loreto 03.40 S, 77.21 W + Rivero (1968) Tambopata, Depto. Madre de

Dios 12.44 S, 69.11 W + MUSN Teniente López, Depto. Loreto 02.36 S, 76.07 W − Duellman and Mendelson (1995) Yurimaguas, Depto. Loreto 05.54 S, 76.05 W − Authors’ pers. observ Suriname (4 localities, 3 presences) Brownsberg 04.55 N, 55.10 W + AMNH, KU Corentijne River 05.10 N, 57.20 W − S. Reichle, pc Monts Tumuc-Humac 02.20 N, 54.40 W + Lescure (1976, 1981a) Mt. Kasikasima 03.00 N, 55.30 W + MZUSP Venezuela (1 locality, 0 presence) Cerro Duida, Edo. Amazonas 03.30 N, 65.40 W     As an altitudinal limit 800 m above sea level was chosen here (i.e. the approximate upper border of the tierra caliente lowlands). Localities in this list from where samples were used for molecular analyses are marked by an asterisk.