Histopathology scores from the serial passage experiment were further analyzed using the Mantel test for trends with correction for continuity
; for this test, data were cast in a two-way table for each C. jejuni strain according to the number of the serial passage of the strain and the Everolimus molecular weight number of animals exhibiting lesions of grades 0 and 1 combined (scores ≤ 19) compared to the number of animals exhibiting lesions of grade 2 (scores ≥ 20). The choice to divide the data in this way for this analysis was made because almost all of the medians of the histopathology scores of C57BL/6 IL-10-/-mice infected with non-adapted C. jejuni 11168 for 28–35 days in previous experiments fell into grade 1 (median scores between 9.5 and 19;  and unpublished Selleck Rapamycin data), whereas the median scores of mice infected with serially passaged C. jejuni 11168 all fell into grade 2. ELISA data were transformed as previously described  prior to analysis by one or two-way ANOVA with post hoc tests using SigmaStat 3.1. GACK analysis was performed on the microarray data using programs available at http://falkow.stanford.edu/whatwedo/software/software.html.
Acknowledgements This project was funded in whole with federal funds from NIAID, NIH, Department of Health and Human Services, under Contract No. N01-AI-30058. We thank Patricia Fields
of the Enteric Diseases Reference Labs, Centers for Disease Control for strains D0121, D0835, D2586, and D2600. We thank Jodi Parrish and Russ Finley of Wayne State University for the contribution of the PCR primers for C. jejuni 11168 ORFs and David Dewitt of MSU for sequencing of the ORFs. We thank Jeff Landgraf of the MSU RTSF for advice and technical assistance with the microarray experiment. We thank Kathleen Campbell, Amy Porter, and Rick Rosebury of the MSU Investigative Histopathology Laboratory for excellent histopathology services. Dr. Rathinam’s salary was PLX3397 chemical structure provided by matching CYTH4 funds from the MSU-CVM. This publication made use of the Campylobacter Multi Locus Sequence Typing website http://pubmlst.org/campylobacter/ developed by Keith Jolley and Man-Suen Chan  and sited at the University of Oxford. Initial development of this site was funded by the Wellcome Trust; maintenance is funded by DEFRA. Electronic supplementary material Additional file 1: Table S1. C. jejuni colonization status of mice at necropsy. The data provided show the percent of mice in which C. jejuni was detected at necropsy by culture or by PCR assay in feces and four sites in the gastrointestinal tract in experiments reported in the main text. Table S2. Genes present in strain 11168 but confirmed absent or strongly divergent in strain NW.